Long non-coding RNA PCAT-1 contributes to tumorigenesis by regulating FSCN1 via miR-145-5p in prostate cancer

Biomed Pharmacother. 2017 Nov;95:1112-1118. doi: 10.1016/j.biopha.2017.09.019. Epub 2017 Oct 6.

Abstract

Prostate cancer associated lncRNA transcript 1 (PCAT-1) has been identified as an oncogenic long non-coding RNA (lncRNA) in some solid tumors, including prostate cancer (PC). However, the molecular mechanism of PCAT-1 involved in PC is poorly defined. In this study, we found that PCAT-1 expression was up-regulated and miR-145-5p expression was down-regulated in PC tissues and cells. Function analysis indicated that PCAT-1 overexpression promoted proliferation, migration, invasion and inhibited apoptosis of PC cells. Rescue experiments demonstrated that miR-145-5p restoration attenuated the promotive effects of PCAT1 on PC progression, while Fascin-1 (FSCN1) upregulation relieved the anti-cancer role of miR-145-5p in PC. Mechanical analysis discovered that PCAT-1 could act as a miR-145-5p sponge to modulate FSCN1 expression. In conclusion, these findings suggested that PCAT-1 accelerated PC cell proliferation, migration, invasion and suppressed apoptosis by up-regulating FSCN1 mediated via miR-145-5p, hinting a potential therapeutic strategy for PC patients.

Keywords: FSCN1; Prostate cancer; lncRNA PCAT-1; miR-145-5p.

MeSH terms

  • Apoptosis / genetics
  • Base Sequence
  • Carcinogenesis / genetics*
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Caspase 3 / metabolism
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Disease Progression
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Microfilament Proteins / genetics*
  • Microfilament Proteins / metabolism
  • Neoplasm Invasiveness
  • Prostatic Neoplasms / enzymology
  • Prostatic Neoplasms / genetics*
  • Prostatic Neoplasms / pathology*
  • RNA, Long Noncoding / genetics
  • RNA, Long Noncoding / metabolism*
  • Reproducibility of Results

Substances

  • Carrier Proteins
  • FSCN1 protein, human
  • MIRN145 microRNA, human
  • MicroRNAs
  • Microfilament Proteins
  • RNA, Long Noncoding
  • long non-coding RNA PCAT1, human
  • Caspase 3