d-limonene ameliorates diabetes and its complications in streptozotocin-induced diabetic rats

Food Chem Toxicol. 2017 Dec;110:434-442. doi: 10.1016/j.fct.2017.09.020. Epub 2017 Sep 18.

Abstract

It is known that diabetes causes some complications including alterations in lipid profile, hepatic enzyme levels but also it causes oxidative stress. Limonene, a major component of Citrus oils, has important health beneficial effects in lowering the level of oxidative stress due to its antioxidant activity. The aim of this study was to investigate the effects of D-limonene on streptozotocin (STZ)-induced diabetes in Wistar albino rats. For this purpose, DNA damage was evaluated by alkaline comet assay. Changes in the activities of catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR) and glutathione peroxidase (GSHPx) and the levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG), total glutathione (GSH), malondialdehyde (MDA), insulin, total bilirubin and BCA protein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and gamma-glutamyl transferase (GGT), high density lipoprotein (HDL), low density lipoprotein (LDL), total cholesterol and triglyceride were also evaluated. D-limonene treatment was found to significantly decrease DNA damage, GR enzyme activities and MDA levels and significantly increase GSH levels and CAT, SOD and GSH-Px enzyme activities and altered lipid and liver enzyme parameters in diabetic rats. According to our results, it seems that D-limonene might have a role in the prevention of the complication of diabetes in rats.

Keywords: DNA damage; Diabetes; Hepatic enzymes; Lipid profil; Oxidative stress.

MeSH terms

  • Animals
  • Aspartate Aminotransferases / metabolism
  • Catalase / metabolism
  • Citrus / chemistry*
  • Cyclohexenes / administration & dosage*
  • Cyclohexenes / chemistry
  • DNA Damage / drug effects
  • Diabetes Mellitus, Experimental / complications
  • Diabetes Mellitus, Experimental / drug therapy*
  • Diabetes Mellitus, Experimental / genetics
  • Diabetes Mellitus, Experimental / metabolism
  • Female
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Humans
  • Limonene
  • Lipoproteins, HDL / metabolism
  • Lipoproteins, LDL / metabolism
  • Liver / drug effects
  • Liver / enzymology
  • Liver / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects
  • Plant Extracts / administration & dosage*
  • Plant Extracts / chemistry
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Terpenes / administration & dosage*
  • Terpenes / chemistry

Substances

  • Cyclohexenes
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Plant Extracts
  • Terpenes
  • Malondialdehyde
  • Limonene
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase
  • Aspartate Aminotransferases
  • Glutathione