Generation of a KLF15 homozygous knockout human embryonic stem cell line using paired CRISPR/Cas9n, and human cardiomyocytes derivation

Stem Cell Res. 2017 Aug;23:127-131. doi: 10.1016/j.scr.2017.07.007. Epub 2017 Jul 11.


Krueppel-like factor 15 (KLF15) is abundantly expressed in liver, kidney, and muscle, including myocardium. In the adult heart KLF15 is important to maintain homeostasis and to repress hypertrophic remodeling. We generated a homozygous hESC KLF15 knockout (KO) line using paired CRISPR/Cas9n. KLF15-KO cells maintained full pluripotency and differentiation potential as well as genomic integrity. We demonstrated that KLF15-KO cells can be differentiated into morphologically normal cardiomyocytes turning them into a valuable tool for studying human KLF15-mediated mechanisms resulting in human cardiac dysfunction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems / genetics*
  • Cell Culture Techniques / methods*
  • Cell Line
  • Gene Knockout Techniques*
  • Homozygote
  • Human Embryonic Stem Cells / cytology*
  • Human Embryonic Stem Cells / metabolism*
  • Humans
  • Kruppel-Like Transcription Factors / genetics*
  • Myocytes, Cardiac / cytology*
  • Nuclear Proteins / genetics*


  • KLF15 protein, human
  • Kruppel-Like Transcription Factors
  • Nuclear Proteins