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. 2017 Oct 20;12(10):2546-2551.
doi: 10.1021/acschembio.7b00647. Epub 2017 Sep 21.

Live Cell Imaging of Mitochondrial Autophagy With a Novel Fluorescent Small Molecule

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Live Cell Imaging of Mitochondrial Autophagy With a Novel Fluorescent Small Molecule

Hidefumi Iwashita et al. ACS Chem Biol. .

Abstract

There has been a growing interest in mitophagy, mitochondria-selective autophagy, which plays an essential role in maintaining intracellular homeostasis. We have developed a small-molecule fluorescent probe, Mtphagy Dye, for visualizing mitophagy, which was readily synthesized from a known perylene derivative, perylene-3,4-dicarboxylic anhydride. Mtphagy Dye has suitable fluorescent properties for detecting mitochondrial acidification during mitophagy in the long-wavelength region that does not damage mitochondria. Using Mtphagy Dye, we were able to visualize mitophagy with both cases of Parkin-dependent and -independent HeLa cells.

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