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. 2017 Sep 28;53(78):10824-10826.
doi: 10.1039/c7cc06409h.

Polymer Nanodiscs and Macro-Nanodiscs of a Varying Lipid Composition

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Polymer Nanodiscs and Macro-Nanodiscs of a Varying Lipid Composition

Venkata Sudheer Kumar Ramadugu et al. Chem Commun (Camb). .
Free PMC article

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Abstract

Polymer lipid nanodiscs have enabled some exciting structural biology and nanobiotechnology applications. The use of a small molecular weight polymer (SMA-EA) has been demonstrated to dramatically increase the size of nanodiscs (up to ∼60 nm diameter). Here, we report the first demonstration of the formation of macro-nanodiscs for a variety of lipids, and solid-state NMR experiments utilizing their magnetic-alignment properties.

Figures

Figure 1
Figure 1
(A) Structure of SMA-EA polymer, (B) Schematic showing a SMA-EA nanodisc containing a lipid bilayer, (C) TEM micrograph of DMPC macro-nanodisc with 1:1 (w/w) ratio of DMPC:SMA-EA, (D) DLS profiles of macro-nanodiscs of 1:1 lipid:polymer molar ratio (also see Figure S1), and (E) 31P solid-state NMR spectra of MLVs and the isotropic nanodiscs formed with the addition of polymer as indicated by lipid:polymer (w/w). Solubilization of 7:3 POPC: POPG MLVs by SMA-EA to form nanodiscs revealed by 31P NMR is shown in Figure S2.
Figure 2
Figure 2
Magnetic alignment of 1:1 DMPC:SMA-EA macro-nanodiscs in a fluid lamellar phase obtained at 35 °C with the addition of indicated amount of lanthanide salt (YbCl3). 31P (A) and 14N (C) NMR spectra. Plot of 31P chemical shifts (C) and 14N quadrupolar coupling (D) measured from the DMPC-SMA-EA macro-nanodiscs for different concentrations of YbCl3. 31P and 14N spectra of macro-nanodiscs for varying concentration of YbCl3 are shown in Figures S3 and S4.
Figure 3
Figure 3
31P (top) and 14N (bottom) NMR spectra revealing the magnetic-alignment of SMA-EA polymer macro-nanodiscs composed of POPC, DPPC or DSPC. The gel to liquid crystalline phase transition temperatures for POPC, DPPC and DSPC are −2, 41 and 55 °C, respectively.
Figure 4
Figure 4
Structure of DMPC (A), 2D 1H-13C PELF spectrum of 1:1 DMPC:SMA-EA macro-nanodiscs (B) recorded at 35 °C, and 1H-13C dipolar coupling spectral slices extracted for different carbons of DMPC (C) 2D PELF spectrum of 1:1 DPPC: SMA-EA macro-nanodiscs is given in Figure S5.

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