Upregulation of miR-520b promotes ovarian cancer growth

Oncol Lett. 2017 Sep;14(3):3155-3161. doi: 10.3892/ol.2017.6552. Epub 2017 Jul 8.


Ovarian cancer is the most common gynecological malignant cancer in female genitalia. Dysregulation or dysfunction of microRNAs (miRs) contribute to cancer development. The role of miR-520b in ovarian cancer remains unclear. The present study investigated the role of miR-520b in ovarian cancer and determined that the expression levels of miR-520b in ovarian cancer tissues and cell lines were upregulated. By contrast, reverse transcription-quantitative polymerase chain reaction and immunohistochemistry revealed that the mRNA and protein expression levels of ring finger protein 216 (RNF216) were downregulated in ovarian cancer, indicating that there was a negative correlation between miR-520b and RNF216. In miR-520b-knockdown cells, downregulation of miR-520b reduced cell proliferation, while upregulation of miR-520b promoted cell proliferation. In addition, RNF216 was predicted by TargetScanHuman and was observed to be targeted by miR-520b. In conclusion, the present data indicated that high expression of miR-520b in ovarian cancer promoted cell growth via RNF216.

Keywords: RNF216; cancer growth; miR-520b; ovarian cancer.