Rapid whole brain imaging of neural activity in freely behaving larval zebrafish ( Danio rerio)

Elife. 2017 Sep 20;6:e28158. doi: 10.7554/eLife.28158.


The internal brain dynamics that link sensation and action are arguably better studied during natural animal behaviors. Here, we report on a novel volume imaging and 3D tracking technique that monitors whole brain neural activity in freely swimming larval zebrafish (Danio rerio). We demonstrated the capability of our system through functional imaging of neural activity during visually evoked and prey capture behaviors in larval zebrafish.

Keywords: 3D tracking system; freely behaving larval zebrafish; light field microscope; neuroscience; prey capture behavior; whole brain Imaging; zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Behavior, Animal
  • Brain / physiology
  • Imaging, Three-Dimensional / methods
  • Larva / physiology
  • Neuroimaging / methods*
  • Zebrafish / physiology*

Grant support

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.