Panduratin A Prevents Tumor Necrosis Factor-Alpha-Induced Muscle Atrophy in L6 Rat Skeletal Muscle Cells

J Med Food. 2017 Nov;20(11):1047-1054. doi: 10.1089/jmf.2017.3970. Epub 2017 Sep 21.


Panduratin A, a prenylated chalcone compound, has anti-obesity, anti-bacterial, anti-cancer, anti-inflammatory, and anti-oxidative activities. However, its preventive effect on muscle atrophy has not been studied. The purpose of this study was to evaluate the inhibitory effect of panduratin A on muscle atrophy and to investigate its molecular mechanisms in tumor necrosis factor-alpha (TNF-α)-treated L6 rat skeletal muscle cells. Panduratin A restored the myotube diameter reduced by TNF-α. At the molecular level, panduratin A elevated phosphatidylinositol 3 kinase/Akt/mammalian target of rapamycin pathway and stimulated the MyoD and myogenin mRNA expression decreased by TNF-α. However, panduratin A attenuated the mRNA expression of E3 ubiquitin ligase and autophagy-related genes. Moreover, panduratin A significantly inhibited reactive oxygen species production by increasing the mRNA expression of catalase and superoxide dismutase. Overall, panduratin A might be a useful agent for the treatment of muscle atrophy.

Keywords: TNF-α; inflammation; muscle atrophy; panduratin A.

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Cell Line
  • Chalcones / pharmacology*
  • Humans
  • Muscle Cells / cytology
  • Muscle Cells / drug effects*
  • Muscle Cells / metabolism
  • Muscle, Skeletal / cytology*
  • Muscle, Skeletal / drug effects
  • Muscle, Skeletal / metabolism
  • Muscular Atrophy / drug therapy
  • Muscular Atrophy / genetics
  • Muscular Atrophy / metabolism*
  • Muscular Atrophy / physiopathology
  • Plant Extracts / administration & dosage*
  • Rats
  • Rhizome / chemistry
  • Signal Transduction / drug effects
  • Tumor Necrosis Factor-alpha / metabolism*
  • Zingiberaceae / chemistry*


  • Chalcones
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • panduratin A