[A new in vitro model allows use of the same hypothalamus for morphological and functional studies]

Ann Endocrinol (Paris). 1987;48(5):438-40.
[Article in French]


A model for combined morphological and functional investigations on the isolated rat mediobasal hypothalamus has been developed. Under these conditions of incubation, the hypothalamic tissue is well preserved, on the basis of photonic and electronic microscopic examinations. This model has been used to study the LHRH system in the rat. LHRH release has been measured in the incubation medium under basal conditions and after KCl-induced depolarisation; the, LHRH has been localized by immunohistochemistry on the hypothalamic fragment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electrophysiology
  • Gonadotropin-Releasing Hormone / analysis
  • Gonadotropin-Releasing Hormone / metabolism
  • Hypothalamus, Middle* / anatomy & histology
  • Hypothalamus, Middle* / drug effects
  • Hypothalamus, Middle* / physiology
  • Immunohistochemistry
  • In Vitro Techniques
  • Male
  • Potassium Chloride / pharmacology
  • Rats
  • Rats, Inbred Strains
  • Somatostatin / analysis
  • Thyrotropin-Releasing Hormone / analysis


  • Gonadotropin-Releasing Hormone
  • Somatostatin
  • Thyrotropin-Releasing Hormone
  • Potassium Chloride