Antigen CD34+ marrow cells engraft lethally irradiated baboons

J Clin Invest. 1988 Mar;81(3):951-5. doi: 10.1172/JCI113409.


The CD34 antigen is present on 1-4% of human marrow cells including virtually all hematopoietic progenitors detected by in vitro assays. Since the anti-CD34 monoclonal antibody 12-8 reacts with a similar marrow population in baboons, it was possible to test whether this antigen is expressed by stem cells responsible for hematopoietic reconstitution in vivo. CD34+ cells were enriched from marrows of five baboons using avidin-biotin immunoadsorption. After lethal irradiation, the five animals were given 15-27 X 10(6) autologous marrow cells (3.2-4.4 X 10(6) cells/kg) containing 65-91% CD34+ cells. All animals achieved granulocyte counts greater than 1,000/mm3 and platelet counts greater than 20 X 10(3)/mm3 by 13-24 d posttransplant and subsequently developed normal peripheral blood counts. Two additional animals received 184 and 285 X 10(6) marrow cells/kg depleted of CD34+ cells. One animal died at day 29 without engraftment, while the other had pancytopenia for greater than 100 d posttransplant. The data suggest that stem cells responsible for hematopoietic reconstitution are CD34+.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Differentiation*
  • Bone Marrow / pathology
  • Bone Marrow Transplantation*
  • Cell Separation
  • Graft Survival*
  • Hematopoietic Stem Cell Transplantation*
  • Hematopoietic Stem Cells / classification
  • Hematopoietic Stem Cells / pathology
  • Papio
  • Phenotype
  • Postoperative Complications / pathology
  • Radiation Chimera*
  • Syndrome


  • Antigens, Differentiation