The long noncoding RNA HOTAIR activates the Hippo pathway by directly binding to SAV1 in renal cell carcinoma

Guanghui Hu; Binbin Dong; Jingwei Zhang; Wei Zhai; Tiancheng Xie; Bisheng Huang; Chi Huang; Xudong Yao; Junhua Zheng; Jianping Che; Yun-Fei Xu

doi:10.18632/oncotarget.17414

The long noncoding RNA HOTAIR activates the Hippo pathway by directly binding to SAV1 in renal cell carcinoma

Oncotarget. 2017 Apr 25;8(35):58654-58667. doi: 10.18632/oncotarget.17414. eCollection 2017 Aug 29.

Authors

Guanghui Hu^#¹, Binbin Dong^#², Jingwei Zhang^#¹, Wei Zhai^{1

3}, Tiancheng Xie¹, Bisheng Huang¹, Chi Huang⁴, Xudong Yao¹, Junhua Zheng¹, Jianping Che^{1

4}, Yun-Fei Xu¹

Affiliations

¹ Department of Urology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
² Department of Urology, Yancheng Third People's Hospital, Yancheng, Jiangsu, China.
³ Department of Urology, Renji Hospital, Shanghai Jiaotong University, Shanghai, China.
⁴ Department of Urology, Shanghai Tenth People's Hospital, Nanjing Medical University, Nanjing, Jiangsu, China.

^# Contributed equally.

Abstract

The long noncoding RNA HOTAIR promotes the development and progression of several tumors. Here, the clinical significance and role of HOTAIR in renal cell carcinoma (RCC) tumorigenesis were explored. The results showed that increased expression of HOTAIR predicted a poor prognosis of RCC after surgery. HOTAIR promoted RCC cell proliferation and growth in vitro and in vivo. The expressions of HOTAIR and Salvador homolog 1 (SAV1) were inversely correlated in clinical RCC samples. HOTAIR downregulated SAV1 by directly binding to the SAV1 protein and enhanced histone H3K27 methylation. Loss of function of SAV1 activated the Hippo pathway. HOTAIR could be a potential therapeutic target in RCC.

Keywords: HOTAIR; SAV1; hippo pathway; renal cell carcinoma.