Luciferases with Tunable Emission Wavelengths

Angew Chem Int Ed Engl. 2017 Nov 13;56(46):14556-14560. doi: 10.1002/anie.201708277. Epub 2017 Oct 9.

Abstract

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

Keywords: bioluminescent imaging; luciferase; self-labeling protein; sensors; therapeutic drug monitoring.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques
  • Fluorescent Dyes / chemistry
  • Genes, Reporter
  • HeLa Cells
  • Humans
  • Luciferases / chemistry*
  • Luminescent Measurements / methods

Substances

  • Fluorescent Dyes
  • Luciferases