A single administration of fish oil inhibits the acute inflammatory response in rats

Laura Lícia Milani de Arruda; Franciele Queiroz Ames; Damila Rodrigues de Morais; Renata Grespan; Ana Paula Maziero Gil; Maria Angélica Raffaini Covas Pereira Silva; Jesuí Vergílio Visentainer; Roberto Kenji Nakamura Cuman; Ciomar Aparecida Bersani-Amado

doi:10.1016/j.apjtm.2017.07.019

A single administration of fish oil inhibits the acute inflammatory response in rats

Asian Pac J Trop Med. 2017 Aug;10(8):765-772. doi: 10.1016/j.apjtm.2017.07.019. Epub 2017 Aug 22.

Authors

Laura Lícia Milani de Arruda¹, Franciele Queiroz Ames¹, Damila Rodrigues de Morais², Renata Grespan¹, Ana Paula Maziero Gil¹, Maria Angélica Raffaini Covas Pereira Silva¹, Jesuí Vergílio Visentainer², Roberto Kenji Nakamura Cuman¹, Ciomar Aparecida Bersani-Amado³

Affiliations

¹ Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil.
² Department of Chemistry, State University of Maringá, Maringá, PR, Brazil.
³ Department of Pharmacology and Therapeutics, State University of Maringá, Maringá, PR, Brazil. Electronic address: cabamado@uem.br.

PMID: 28942825
DOI: 10.1016/j.apjtm.2017.07.019

Abstract

Objective: To investigate the antiinflammatory effects of a single administration of fish oil (FO) on the acute inflammatory response.

Methods: The paw edema and pleurisy models were used to evaluate the effects of FO dissolved in olive oil (FOP) orally administered in a single dose in rats. Nitric oxide (NO) concentrations in the pleural exudate were performed according to the Griess method and the cytokine concentrations were determined by Luminex bead-based multiplex assay.

Results: FOP treatment (30 and 300 mg/kg) significantly reduced paw edema. FOP treatment at 18.75, 37.5, 75.0, 150.0, and 300 mg/kg decreased both the volume of pleural exudate and cellular migration into the pleural cavity and each of these doses presented the same effectiveness. Treatment with FOP (300 mg/kg) reduced NO, TNF-α, IL-1β, and IL-6 concentrations in the pleural exudate.

Conclusions: The present data provide evidence that FO has inhibitory effects on the acute inflammatory response when administered in a single dose in rats. This effect might be attributable to a direct inhibitory effect of FO on the production or release of inflammatory mediators that are involved in the pathological processes evaluated herein.

Keywords: DHA; EPA; Leukocyte migration; Omega-3 fatty acid; Pleurisy; Polyunsaturated fatty acid.