[DNA marker-assisted selection of medicinal plants (Ⅰ) .Breeding research of disease-resistant cultivars of Panax notoginseng]

Zhongguo Zhong Yao Za Zhi. 2017 Jan;42(1):70-75. doi: 10.19540/j.cnki.cjcmm.20161222.039.
[Article in Chinese]

Abstract

The study is aimed to estimate the diversity of arbuscular mycorrhizal fungi (AMF) in the main producing areas of Salvia miltiorrhiza.Diversity of AMF was surveyed directly on spores isolated from the field soil, collected from 20 sites of 8 provinces. Identification of the AMF was made by observation of spore morphology. At least 27 recognized AMF species were identified in samples from field soil, belonging to seven genera of AMF-Acaulospora, Glomus, Funneliformis, Ambispora, Rhizophagus, Pacispora, and Claroideoglomus. Acaulospora and Glomus were the dominant genera, respectively including nine and eight species. A. laevis (90%), R.manihotis (80%), A. brieticulata (75%), A. tuberculata (70%) were the dominant species.Colonization rate was determined,colonization was easily found, but the colonization intensities were low, the colonization rate remained at 10.92%-25.93%. The similarity between provinces is generally low, and the similarity coefficients were from 0.20 to 0.57.

Keywords: Salvia miltiorrhiza; arbuscular mycorrhizal fungi; colonization rate; diversity; identification.

MeSH terms

  • Genetic Markers
  • Mycorrhizae / classification*
  • Panax notoginseng / genetics
  • Panax notoginseng / microbiology*
  • Plant Breeding*
  • Plants, Medicinal / genetics
  • Plants, Medicinal / microbiology
  • Selection, Genetic
  • Soil Microbiology*

Substances

  • Genetic Markers