Edaphobacter acidisoli sp. nov., an acidobacterium isolated from forest soil

Int J Syst Evol Microbiol. 2017 Oct;67(10):4260-4265. doi: 10.1099/ijsem.0.002300. Epub 2017 Sep 25.


Two strains, 4G-K17T and 4G-K15, were isolated from forest soil from the Dinghushan Biosphere Reserve, Guangdong Province, PR China (112° 31' E 23° 10' N). The cells of the two strains were Gram-stain-negative, aerobic and non-motile short rods that multiplied by binary division. Strains 4G-K17T and 4G-K15 were obligately acidophilic, mesophilic bacteria capable of growth at pH 3.0-7.0 (optimum 4.0-5.5 and 3.5-5.5, respectively), temperature 12-42 °C (optimum 28 °C) and NaCl concentrations from 0~2.5 % and 0~3.0 % (w/v), respectively. They had a 16S rRNA gene sequence similarity of 99.2 % and showed the highest similarities of 97.1 and 97.4 % to Edaphobacter aggregans Wbg-1T, respectively, which indicated that these two isolates belonged to a novel species of the genus Edaphobacter in subdivision 1 in the family Acidobacteriaceae. The DNA G+C contents of strains 4G-K17T and 4G-K15 were 57.6 and 57.2 %, respectively. They had similar fatty acid profiles, with the major (>10 %) fatty acid profile comprising iso-C15 : 0, C16 : 0 and summed feature 3 (iso-C16 : 1ω7c and/or C16 : 1ω6c), and the major polar lipid profile comprising phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified aminophospholipids and unidentified phospholipids. All physiological, phylogenetic and chemotaxonomic data suggest that strains 4G-K17T and 4G-K15 belong to a new species of the genus Edaphobacter, for which the name Edaphobacter acidisoli sp. nov. is proposed, with 4G-K17T (=CGMCC 1.15447=LMG 29212) as the type strain.

Keywords: Edaphobacter; acidophilic; phylogeny.

MeSH terms

  • Acidobacteria / classification*
  • Acidobacteria / genetics
  • Acidobacteria / isolation & purification
  • Bacterial Typing Techniques
  • Base Composition
  • China
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Forests*
  • Phospholipids / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*


  • DNA, Bacterial
  • Fatty Acids
  • Phospholipids
  • RNA, Ribosomal, 16S