Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

doi:10.1038/nmeth.4436

. 2017 Nov;14(11):1055-1062.

doi: 10.1038/nmeth.4436. Epub 2017 Sep 25.

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Xiaodong Liu^{1

2

3}, Christian M Nefzger^{1

2

3}, Fernando J Rossello^{1

2

3}, Joseph Chen^{1

2

3}, Anja S Knaupp^{1

2

3}, Jaber Firas^{1

2

3}, Ethan Ford^{4

5}, Jahnvi Pflueger^{4

5}, Jacob M Paynter^{1

2

3}, Hun S Chy^{3

6}, Carmel M O'Brien^{3

6}, Cheng Huang⁷, Ketan Mishra^{1

2

3}, Margeaux Hodgson-Garms^{1

2

3}, Natasha Jansz^{8

9}, Sarah M Williams^{1

2

3

10}, Marnie E Blewitt^{8

9}, Susan K Nilsson^{3

6}, Ralf B Schittenhelm⁷, Andrew L Laslett^{3

6}, Ryan Lister^{4

5}, Jose M Polo^{1

2

3}

Affiliations

¹ Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
² Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
³ Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
⁴ ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, Perth, Western Australia, Australia.
⁵ The Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
⁶ Manufacturing, CSIRO, Clayton, Victoria, Australia.
⁷ Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
⁸ The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
⁹ University of Melbourne, Melbourne, Victoria, Australia.
¹⁰ Monash Bioinformatics Platform, Monash University, Wellington Road, Clayton, Victoria, Australia.

PMID: 28945704
DOI: 10.1038/nmeth.4436

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Xiaodong Liu et al. Nat Methods. 2017 Nov.

. 2017 Nov;14(11):1055-1062.

doi: 10.1038/nmeth.4436. Epub 2017 Sep 25.

Authors

Affiliations

¹ Department of Anatomy and Developmental Biology, Monash University, Wellington Road, Clayton, Victoria, Australia.
² Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Wellington Road, Clayton, Victoria, Australia.
³ Australian Regenerative Medicine Institute, Monash University, Wellington Road, Clayton, Victoria, Australia.
⁴ ARC Center of Excellence in Plant Energy Biology, The University of Western Australia, Perth, Western Australia, Australia.
⁵ The Harry Perkins Institute of Medical Research, Perth, Western Australia, Australia.
⁶ Manufacturing, CSIRO, Clayton, Victoria, Australia.
⁷ Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia.
⁸ The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
⁹ University of Melbourne, Melbourne, Victoria, Australia.
¹⁰ Monash Bioinformatics Platform, Monash University, Wellington Road, Clayton, Victoria, Australia.

PMID: 28945704
DOI: 10.1038/nmeth.4436

Abstract

Recent reports on the characteristics of naive human pluripotent stem cells (hPSCs) obtained using independent methods differ. Naive hPSCs have been mainly derived by conversion from primed hPSCs or by direct derivation from human embryos rather than by somatic cell reprogramming. To provide an unbiased molecular and functional reference, we derived genetically matched naive hPSCs by direct reprogramming of fibroblasts and by primed-to-naive conversion using different naive conditions (NHSM, RSeT, 5iLAF and t2iLGöY). Our results show that hPSCs obtained in these different conditions display a spectrum of naive characteristics. Furthermore, our characterization identifies KLF4 as sufficient for conversion of primed hPSCs into naive t2iLGöY hPSCs, underscoring the role that reprogramming factors can play for the derivation of bona fide naive hPSCs.

PubMed Disclaimer

Cited by

A Comparative Study of Cell Culture Conditions during Conversion from Primed to Naive Human Pluripotent Stem Cells.
Romayor I, Herrera L, Burón M, Martin-Inaraja M, Prieto L, Etxaniz J, Inglés-Ferrándiz M, Pineda JR, Eguizabal C. Romayor I, et al. Biomedicines. 2022 Jun 9;10(6):1358. doi: 10.3390/biomedicines10061358. Biomedicines. 2022. PMID: 35740381 Free PMC article.
Integrated analysis of single-cell embryo data yields a unified transcriptome signature for the human pre-implantation epiblast.
Stirparo GG, Boroviak T, Guo G, Nichols J, Smith A, Bertone P. Stirparo GG, et al. Development. 2018 Feb 7;145(3):dev158501. doi: 10.1242/dev.158501. Development. 2018. PMID: 29361568 Free PMC article.
De novo DNA methyltransferases DNMT3A and DNMT3B are essential for XIST silencing for erosion of dosage compensation in pluripotent stem cells.
Fukuda A, Hazelbaker DZ, Motosugi N, Hao J, Limone F, Beccard A, Mazzucato P, Messana A, Okada C, San Juan IG, Qian M, Umezawa A, Akutsu H, Barrett LE, Eggan K. Fukuda A, et al. Stem Cell Reports. 2021 Sep 14;16(9):2138-2148. doi: 10.1016/j.stemcr.2021.07.015. Epub 2021 Aug 19. Stem Cell Reports. 2021. PMID: 34416176 Free PMC article.
Principles of signaling pathway modulation for enhancing human naive pluripotency induction.
Bayerl J, Ayyash M, Shani T, Manor YS, Gafni O, Massarwa R, Kalma Y, Aguilera-Castrejon A, Zerbib M, Amir H, Sheban D, Geula S, Mor N, Weinberger L, Naveh Tassa S, Krupalnik V, Oldak B, Livnat N, Tarazi S, Tawil S, Wildschutz E, Ashouokhi S, Lasman L, Rotter V, Hanna S, Ben-Yosef D, Novershtern N, Viukov S, Hanna JH. Bayerl J, et al. Cell Stem Cell. 2021 Sep 2;28(9):1549-1565.e12. doi: 10.1016/j.stem.2021.04.001. Epub 2021 Apr 28. Cell Stem Cell. 2021. PMID: 33915080 Free PMC article.
Improved Sendai viral system for reprogramming to naive pluripotency.
Kunitomi A, Hirohata R, Arreola V, Osawa M, Kato TM, Nomura M, Kawaguchi J, Hara H, Kusano K, Takashima Y, Takahashi K, Fukuda K, Takasu N, Yamanaka S. Kunitomi A, et al. Cell Rep Methods. 2022 Oct 17;2(11):100317. doi: 10.1016/j.crmeth.2022.100317. eCollection 2022 Nov 21. Cell Rep Methods. 2022. PMID: 36447645 Free PMC article.

See all "Cited by" articles

References

1. Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):4484-9 - PubMed
1. Stem Cell Reports. 2016 Apr 12;6(4):437-446 - PubMed
1. Stem Cell Reports. 2016 Mar 8;6(3):321-9 - PubMed
1. Cell Stem Cell. 2016 Oct 6;19(4):502-515 - PubMed
1. BMC Bioinformatics. 2011 Aug 04;12:323 - PubMed

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- Nature Publishing Group
Other Literature Sources
- The Lens - Patent Citations Database
- scite Smart Citations

[1] Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):4484-9 - PubMed

[2] Proc Natl Acad Sci U S A. 2014 Mar 25;111(12):4484-9 - PubMed

[3] Stem Cell Reports. 2016 Apr 12;6(4):437-446 - PubMed

[4] Stem Cell Reports. 2016 Apr 12;6(4):437-446 - PubMed

[5] Stem Cell Reports. 2016 Mar 8;6(3):321-9 - PubMed

[6] Stem Cell Reports. 2016 Mar 8;6(3):321-9 - PubMed

[7] Cell Stem Cell. 2016 Oct 6;19(4):502-515 - PubMed

[8] Cell Stem Cell. 2016 Oct 6;19(4):502-515 - PubMed

[9] BMC Bioinformatics. 2011 Aug 04;12:323 - PubMed

[10] BMC Bioinformatics. 2011 Aug 04;12:323 - PubMed

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Affiliations

Comprehensive characterization of distinct states of human naive pluripotency generated by reprogramming

Authors

Affiliations

Abstract

Similar articles

Cited by

References

MeSH terms

LinkOut - more resources

Full Text Sources

Other Literature Sources