Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter plate

Yijing Shao; Amy Hui-Mei Lin

doi:10.1016/j.foodchem.2017.07.083

Improvement in the quantification of reducing sugars by miniaturizing the Somogyi-Nelson assay using a microtiter plate

Food Chem. 2018 Feb 1:240:898-903. doi: 10.1016/j.foodchem.2017.07.083. Epub 2017 Jul 21.

Authors

Yijing Shao¹, Amy Hui-Mei Lin²

Affiliations

¹ Bi-State School of Food Science, University of Idaho and Washington State University, 875 Perimeter Dr. MS 2312, Moscow, ID 83843-2312, USA. Electronic address: shao3447@vandals.uidaho.edu.
² Bi-State School of Food Science, University of Idaho and Washington State University, 875 Perimeter Dr. MS 2312, Moscow, ID 83843-2312, USA. Electronic address: amylin@uidaho.edu.

PMID: 28946357
DOI: 10.1016/j.foodchem.2017.07.083

Abstract

Measuring reducing sugar is a common practice in carbohydrate research, and the colorimetric assay developed by Somogyi and Nelson has a high sensitivity in a broad concentration range. However, the method is time-consuming when analyzing a large number of samples. In this study, a modified Somogyi-Nelson assay with excellent accuracy and sensitivity was developed using a 96-well microplate. This microassay greatly improves the analytic capacity and efficacy of the method.

Keywords: DNS; Reducing sugar; Somogyi-Nelson.

MeSH terms

Biological Assay
Carbohydrates / analysis*
Colorimetry

Substances

Carbohydrates