Para-psychobiotic Lactobacillus gasseri CP2305 ameliorates stress-related symptoms and sleep quality

J Appl Microbiol. 2017 Dec;123(6):1561-1570. doi: 10.1111/jam.13594. Epub 2017 Nov 7.


Aims: To confirm the stress-relieving effects of heat-inactivated, enteric-colonizing Lactobacillus gasseri CP2305 (paraprobiotic CP2305) in medical students taking a cadaver dissection course.

Methods and results: Healthy students (21 males and 11 females) took paraprobiotic CP2305 daily for 5 weeks during a cadaver dissection course. The General Health Questionnaire and the Pittsburgh Sleep Quality Index were employed to assess stress-related somatic symptoms and sleep quality respectively. The aggravation of stress-associated somatic symptoms was observed in female students (P = 0·029). Sleep quality was improved in the paraprobiotic CP2305 group (P = 0·038), particularly in men (P = 0·004). Among men, paraprobiotic CP2305 shortened sleep latency (P = 0·035) and increased sleep duration (P = 0·048). Diarrhoea-like symptoms were also effectively controlled with CP2305 (P = 0·005) in men. Thus, we observed sex-related differences in the effects of paraprobiotic CP2305. In addition, CP2305 affected the growth of faecal Bacteroides vulgatus and Dorea longicatena, which are involved in intestinal inflammation.

Conclusions: CP2305 is a potential paraprobiotic that regulates stress responses, and its beneficial effects may depend on specific cell component(s).

Significance and impact of the study: This study characterizes the effects of a stress-relieving para-psychobiotic in humans.

Keywords: Lactobacillus gasseri CP2305; faecal microbiota; para-psychobiotics; sleep quality; stress-relieving effects.

MeSH terms

  • Adult
  • Feces / microbiology
  • Female
  • Humans
  • Lactobacillus gasseri*
  • Male
  • Probiotics / therapeutic use*
  • Sex Factors
  • Sleep*
  • Stress, Psychological / drug therapy*
  • Students, Medical