Hericium erinaceus polysaccharide facilitates restoration of injured intestinal mucosal immunity in Muscovy duck reovirus-infected Muscovy ducklings

Int J Biol Macromol. 2018 Feb;107(Pt A):1151-1161. doi: 10.1016/j.ijbiomac.2017.09.092. Epub 2017 Sep 23.


To elucidate the effect of Hericium erinaceus polysaccharide (HEP) on the intestinal mucosal immunity in normal and Muscovy duck reovirus (MDRV)-infected Muscovy ducklings, 1-day-old healthy Muscovy ducklings were pretreated with 0.2g/L HEP and/or following by MDRV infection in this study, duodenal samples were respectively collected at 1, 3, 6, 10, 15 and 21day post-infection, tissue sections were prepared for observation of morphological structure and determination of intestinal parameters (villus height/crypt depth ratio, villus surface area) as well as counts of intraepithelial lymphocytes (IELs), goblet cells, mast cells. Additionally, dynamics of secretory immunoglobin A (sIgA), interferon-γ (IFN-γ) and interleukin-4 (IL-4) productions in intestinal mucosa were measured with radioimmunoassay. Results showed that HEP significantly improved intestinal morphological structure and related indexes, and significantly inhibited the reduction of intestinal mucosal IELs, goblet cells and mast cells caused by MDRV infection. Furthermore, HEP significantly increased the secretion of sIgA, IFN-γ and IL-4 to enhance intestinal mucosal immune functions. Our findings indicate that HEP treatment can effectively repair MDRV-caused injures of small intestinal mucosal immune barrier, and improve mucosal immune function in sick Muscovy ducklings, which will provide valuable help for further application of HEP in prevention and treatment of MDRV infection.

Keywords: Duodenum; Hericium erinaceus polysaccharide; Mucosal immunity; Muscovy duck reovirus; Restoration.

MeSH terms

  • Animals
  • Autophagy / drug effects
  • Basidiomycota / chemistry
  • Ducks / immunology
  • Ducks / virology
  • Immunity / drug effects*
  • Immunity / immunology
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / immunology
  • Intestinal Mucosa / virology
  • Polysaccharides / chemistry
  • Polysaccharides / pharmacology*
  • Reoviridae / drug effects
  • Reoviridae / pathogenicity
  • Reoviridae Infections / drug therapy*
  • Reoviridae Infections / veterinary
  • Reoviridae Infections / virology


  • Polysaccharides