Potential contribution of tumor-associated slan+ cells as anti-CSF-1R targets in human carcinoma

J Leukoc Biol. 2018 Mar;103(3):559-564. doi: 10.1189/jlb.1MA0717-300R. Epub 2017 Dec 28.


The precise identification of the types and respective roles of the tumor-associated myeloid cells, which include tumor-associated Mϕs (TAMs), neutrophils, dendritic cells, and myeloid-derived suppressor cells, is under intensive investigation. Although tumor-associated myeloid cells may contribute to tumor cell eradication by virtue of their effector functions, they are retained to fulfill predominantly protumorigenic roles. It follows that depletion of tumor-associated myeloid cells represents one of the currently pursued therapeutic options in advanced malignancies. In that regard, RG7155/emactuzumab, a specific anti-CSF-1R humanized Ab, has been reported recently to deplete CSF-1R+ TAMs, in association with objective clinical responses in patients with advanced cancer. Because RG7155/emactuzumab has also been shown to deplete blood non-classic CD14dim/- CD16++ monocytes, which in large part include the CD16++ slan+ monocytes, we asked whether RG7155/emactuzumab could target tumor-associated slan+ cells. In this study, we confirmed that slan+ cells localize only to metastatic tumor-draining lymph nodes, not to primary tumors or distant metastases in patients with different types of carcinoma. Notably, by cell scoring on serial sections, we found that slan+ cells represent a minor fraction of the total CSF-1R+ cell pool, suggesting that slan+ cells potentially represent minor targets of anti-CSF-1R therapy. Therefore, a protumorigenic role for slan+ cells, such as that of CSF-1R+ TAMs, based on our current data, remains questionable.

Keywords: non-classic CD14dim/−CD16++ monocyte; tumor-associated Mϕ; tumor-draining lymph node.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / pharmacology*
  • Antibodies, Monoclonal, Humanized
  • Cells, Cultured
  • Humans
  • Macrophages / drug effects*
  • Monocytes / drug effects*
  • Receptor, Macrophage Colony-Stimulating Factor / antagonists & inhibitors*
  • Receptor, Macrophage Colony-Stimulating Factor / metabolism
  • Tumor Suppressor Proteins / metabolism*


  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • SECISBP2L protein, human
  • Tumor Suppressor Proteins
  • emactuzumab
  • Receptor, Macrophage Colony-Stimulating Factor