Amelioration of Ethanol-Induced Hepatitis by Magnesium Isoglycyrrhizinate through Inhibition of Neutrophil Cell Infiltration and Oxidative Damage

Mediators Inflamm. 2017;2017:3526903. doi: 10.1155/2017/3526903. Epub 2017 Aug 29.

Abstract

Alcoholic liver disease (ALD) is a leading cause of liver-related morbidity and mortality worldwide. There is no effective treatment to prevent the disease progression. Magnesium isoglycyrrhizinate (MgIG) showed potent anti-inflammatory, antioxidant, and hepatoprotective activities and was used for treating liver diseases in Asia. In this study, we examined whether MgIG could protect mice against alcohol-induced liver injury. The newly developed chronic plus binge ethanol feeding model was used to study the role of MgIG in ALD. Serum liver enzyme levels, H&E staining, immunohistochemical staining, flow cytometric analysis, and real-time PCR were used to evaluate the liver injury and inflammation. We showed that MgIG markedly ameliorated chronic plus binge ethanol feeding liver injury, as shown by decreased serum alanine transaminase and aspartate aminotransferase levels and reduced neutrophil infiltration. The reason may be attributed to the reduced expression of proinflammatory cytokines and chemokines with the treatment of MgIG. The hepatoprotective effect of MgIG was associated with suppression of neutrophil ROS production as well as hepatocellular oxidative stress. MgIG may play a critical role in protecting against chronic plus binge ethanol feeding-induced liver injury by regulating neutrophil activity and hepatic oxidative stress.

MeSH terms

  • Animals
  • Chemokines / metabolism
  • Cytokines / metabolism
  • Ethanol / toxicity*
  • Fatty Liver / drug therapy
  • Fatty Liver / metabolism
  • Flow Cytometry
  • Hepatitis, Alcoholic / drug therapy*
  • Hepatitis, Alcoholic / metabolism
  • Lymphocytes / cytology
  • Lymphocytes / drug effects
  • Lymphocytes / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neutrophils / cytology
  • Neutrophils / drug effects*
  • Neutrophils / metabolism*
  • Oxidative Stress / drug effects
  • Reactive Oxygen Species / metabolism
  • Real-Time Polymerase Chain Reaction
  • Saponins / therapeutic use*
  • Signal Transduction / drug effects
  • Triterpenes / therapeutic use*

Substances

  • 18alpha,20beta-hydroxy-11-oxo-norolean-12-en-3beta-yl-2-O-beta-D-glucopyranurosyl-alpha-D-glucopyranosiduronate magnesium tetrahydrate
  • Chemokines
  • Cytokines
  • Reactive Oxygen Species
  • Saponins
  • Triterpenes
  • Ethanol