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. 2016 Jun 30;7:259-265.
doi: 10.1016/j.bbrep.2016.06.014. eCollection 2016 Sep.

Effect of Dietary Fish Oil on Mouse Testosterone Level and the Distribution of Eicosapentaenoic Acid-Containing Phosphatidylcholine in Testicular Interstitium

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Free PMC article

Effect of Dietary Fish Oil on Mouse Testosterone Level and the Distribution of Eicosapentaenoic Acid-Containing Phosphatidylcholine in Testicular Interstitium

Nobuhiro Zaima et al. Biochem Biophys Rep. .
Free PMC article

Abstract

Low levels of serum testosterone are characteristically associated with diabetes, coronary atherosclerosis, obstructive sleep apnea, rheumatoid arthritis, and chronic obstructive pulmonary disease. Testosterone replacement therapy is effective against many of these disorders, indicating the importance of maintaining a healthy testosterone level. In this study, we investigated the effects of fish oil on murine testosterone metabolism and analyzed the dynamics of relevant lipids in testes by matrix-assisted laser desorption ionization mass spectrometry imaging. Testosterone was upregulated in mice that received fish oil. In the testicular interstitium, eicosapentaenoic acid-containing phosphatidylcholine was distributed characteristically. These data suggest that eicosapentaenoic acid is involved in testosterone metabolism.

Keywords: Eicosapentaenoic acid; Fish oil; Matrix-assisted laser desorption ionization mass spectrometry imaging; Phosphatidylcholine; Testis; Testosterone.

Figures

Fig. 1
Fig. 1
Testosterone levels in dietary and gavage administration conditions in relation to fish oil. Immunohistological staining of anti-testosterone in the dietary administration group and the testosterone-positive area of sections of a testis (a). Scale bars=250 µm. Immunohistological staining of anti-testosterone in the gavage group and the testosterone-positive area of sections of a testis (b). Scale bars=250 µm. The serum testosterone level in the dietary administration group (c). The serum testosterone level in the gavage group (d). The data are presented as mean±SEM, n=5;*P<0.05 compared to the control group.
Fig. 2
Fig. 2
Distribution of phosphatidylcholine (PC) molecular species in murine testes. Optical images of a testis of a mouse from the dietary administration group (a and b). Regions of matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) analysis are highlighted. Distributions of PC(36:5) and PC(36:4) (c–f). A merged image of PC(36:5) and PC(36:4) (g and m). Optical images of a testis of a mouse from the gavage group (i and j). Regions of MALDI-MSI analysis are highlighted. Distributions of PC(36:5) and PC(36:4) (k–n). A merged image of the distributions of PC(36:5) and PC(36:4) (o and p); n=5. Scale bars=500 µm (optical image) and 1000 µm (MALDI-MSI data).
Fig. 3
Fig. 3
Comparison of the phosphatidylcholine PC(36:5) data by using hematoxylin-eosin (H&E) staining. The latter staining of a testis from a mouse fed fish oil (a). A merged image of the distributions of PC(36:5) and PC(36:4) (b). Scale bars=1000 µm. A high-power magnified field of the square region from panel a (c). Arrows indicate the testicular interstitium. A high-power magnified field of the square region from panel b (d). Arrows show the testicular interstitium; n=3. Scale bars=200 µm.

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