Statement of problem: The casein phospho peptide-amorphous calcium phosphate with or without fluoride (CPP-ACPF and CPP-ACP respectively) are of considerably new materials which are highly recommended for prevention of dental caries. However, there is a shortage in literature on how they affect the ion concentration of saliva or dental plaque.
Objectives: The aim of this study was to evaluate the concentration of calcium, phosphate and fluoride in the plaque and saliva of children with Early Childhood Caries (ECC) after applying the CPP-ACP paste in comparison with the use of CPP-ACPF paste.
Materials and methods: One ml of un-stimulated saliva of 25 preschool children was collected and then 1 mg of the plaque sample was collected from the buccal surfaces of the two first primary molars on the upper jaw. CPP-ACP as well as CPP-ACPF pastes were applied on the tooth surfaces in two separate steps. In steps, plaque and saliva sampling was performed after 60 minutes. The amount of calcium ions was measured by Atomic Absorption Device and the amount of phosphate and fluoride ions was measured by Ion Chromatography instrument. Data were analyzed using Repeated Measurements ANOVA at a p < 0.05 level of significance.
Results: Application of both CPP-ACPF and CPP-ACP significantly increased the concentration of calcium, phosphate, and fluoride in both saliva and dental plaque. Moreover, significantly higher salivary fluoride concentration was seen after application of CPP-ACPF compared to CPP-ACP. No other significant difference was observed between these two materials.
Conclusions: CPP-ACPF can be more useful than CPP-ACP in protecting the primary teeth against caries process, especially when there is poor hygiene.
Keywords: Casein Phospho Peptide-Amorphous Calcium Phos-phate Fluoride; Saliva; Dental Plaque.
Conflict of interest statement
Conflict of interests: None declared.
Concentration of Calcium, Phosphate and Fluoride Ions in Microbial Plaque and Saliva after Using CPP-ACP Paste in 6-9 year-old Children.J Dent Biomater. 2016 Jun;3(2):214-219. J Dent Biomater. 2016. PMID: 28959745 Free PMC article.
Remineralizing potential of various agents on dental erosion.J Oral Biol Craniofac Res. 2014 May-Aug;4(2):104-8. doi: 10.1016/j.jobcr.2014.05.001. Epub 2014 Aug 21. J Oral Biol Craniofac Res. 2014. PMID: 25737926 Free PMC article.
An in vitro comparison of casein phosphopeptide-amorphous calcium phosphate paste, casein phosphopeptide-amorphous calcium phosphate paste with fluoride and casein phosphopeptide-amorphous calcium phosphate varnish on the inhibition of demineralization and promotion of remineralization of enamel.J Indian Soc Pedod Prev Dent. 2017 Oct-Dec;35(4):312-318. doi: 10.4103/JISPPD.JISPPD_308_16. J Indian Soc Pedod Prev Dent. 2017. PMID: 28914243
Anticariogenicity of casein phosphopeptide-amorphous calcium phosphate: a review of the literature.J Contemp Dent Pract. 2009 May 1;10(3):1-9. J Contemp Dent Pract. 2009. PMID: 19430620 Review.
Review of casein phosphopeptides-amorphous calcium phosphate.Chin J Dent Res. 2014;17(1):7-14. Chin J Dent Res. 2014. PMID: 25028684 Review.
- Featherstone JD. The continuum of dental caries-evidence for a dynamic disease process. J Dent Res. 2004;83:39–42. - PubMed
- Reynolds E. Calcium phosphate-based remineralization systems: scientific evidence? . Aust Dent J. 2008;53:268–273. - PubMed
- Tenovuo J. Antimicrobial function of human saliva-how important is it for oral health? . Acta Odontol Scand. 1998;56:250–256. - PubMed
- Reynolds EC, Cai F, Cochrane NJ, et al. Fluoride and casein phosphopeptide-amorphous calcium phosphate. J Dent Res. 2008;87:344–348. - PubMed