Bovine liver cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex

Biochem Biophys Res Commun. 1988 Apr 15;152(1):264-9. doi: 10.1016/s0006-291x(88)80709-5.

Abstract

cDNA clones encoding a precursor of the alpha-subunit of the mitochondrial ATP synthase complex have been isolated from a bovine liver cDNA library using the alpha-subunit gene from Saccharomyces cerevisiae as a probe. Analyses of the nucleotide sequence of these cDNA clones reveal that the bovine liver ATP synthase alpha-subunit is initially synthesized as a precursor with an aminoterminal extension 43 amino acids in length. This aminoterminal presequence contains seven basic residues, no acidic residues, and seven polar uncharged serine and threonine residues. A single mRNA species, approximately 1.85 kb in length, was detected for the ATP synthase alpha-subunit precursor in both bovine liver and heart.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular
  • DNA / genetics
  • Genes
  • Genes, Fungal
  • Liver / enzymology
  • Macromolecular Substances
  • Mitochondria, Liver / enzymology*
  • Molecular Sequence Data
  • Proton-Translocating ATPases / genetics*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics

Substances

  • Macromolecular Substances
  • DNA
  • Proton-Translocating ATPases

Associated data

  • GENBANK/M19680