Impact of cellular health conditions on the protein folding state in mammalian cells

Chem Commun (Camb). 2017 Oct 10;53(81):11245-11248. doi: 10.1039/c7cc06004a.

Abstract

By using in-cell NMR experiments, we have demonstrated that the protein folding state in cells is significantly influenced by the cellular health conditions. hAK1 was denatured in cells under stressful culture conditions, while it remained functional and properly folded in cells continuously supplied with a fresh medium.

MeSH terms

  • Adenylate Kinase / chemistry*
  • Adenylate Kinase / metabolism
  • HeLa Cells
  • Humans
  • Nuclear Magnetic Resonance, Biomolecular
  • Protein Folding* / drug effects

Substances

  • Adenylate Kinase
  • adenylate kinase 1