A zebrafish engrailed-like homeobox sequence expressed during embryogenesis

FEBS Lett. 1988 Apr 25;231(2):355-60. doi: 10.1016/0014-5793(88)80849-4.


The zebrafish genome was found to contain two sequences which cross-hybridize strongly with the engrailed gene of Drosophila. Several independent clones containing one of these cross-hybridizing sequences were isolated from a zebrafish genomic library. Characterization of this region (ZF-EN) by DNA sequencing showed that it shares about 70% sequence identity with the engrailed homeobox. More extensive homeobox homology (greater than 90%) was found relative to the murine En genes. The closest relationship exists between ZF-EN and En-2 where the C-terminal domains (104 amino acids) encoded by these genes are almost identical. We also observed that ZF-EN and En-2 are very similar with respect to their transcript sizes and temporal expression patterns.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cyprinidae / genetics*
  • DNA, Recombinant
  • Drosophila melanogaster / genetics
  • Embryonic and Fetal Development
  • Gene Expression Regulation
  • Genes, Homeobox*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Sequence Homology, Nucleic Acid
  • Zebrafish / embryology
  • Zebrafish / genetics*


  • DNA, Recombinant

Associated data

  • GENBANK/X07622