Protective effect of Schisandra chinensis bee pollen extract on liver and kidney injury induced by cisplatin in rats

Biomed Pharmacother. 2017 Nov;95:1765-1776. doi: 10.1016/j.biopha.2017.09.083. Epub 2017 Oct 6.


Cisplatin (CP) has been used to cure numerous forms of cancers effectively in clinics, however, it could induce some toxic effects. Bee pollen is a natural compound, produced by honey bees. It is obtained from collected flower pollen and nectar, mixed with bee saliva. Bee pollen produced from Schisandra chinensis plants is described to exert potent antioxidant effects and to be a free radical scavenger. The purpose of this study was to investigate the effects of therapeutic treatment with Schisandra chinensis bee pollen extract (SCBPE) on liver and kidney injury induced by CP. The rats were intragastrically administrated with different doses of SCBPE (400mg/kg/day, 800mg/kg/day, 1200mg/kg/day) and vitamin C (400mg/kg/day, positive control group) for 12days, and the liver and kidney injury models were established by single intraperitoneal injection of CP (8mg/kg) at seventh day. The effect of SCBPE on CP toxicity was evaluated by measuring markers of liver and kidney injury in serum, levels of lipid peroxidation and antioxidants in liver and kidney, observing pathological changes of tissue, and quantified expression of NFκB, IL-1β, IL-6, cytochrome C, caspase3, caspase9, p53 and Bax in liver and kidney. Compared with the model group, the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and the content of blood urea nitrogen (BUN), creatinine (Cr) in serum all decreased in SCBPE high dose group. Meanwhile, the activities of superoxide dismutase (SOD), catalase (CAT) and the content of reduced glutathione (GSH) in liver and kidney increased, and the content of malondialdehyde (MDA) and inducible nitric oxide synthase (iNOS) decreased. In addition, the histopathologic aspects showed that the pathological changes of liver and kidney were found in the model group, and SCBPE group reduced to varying degrees. Moreover, the expression of NFκB, IL-1β, IL-6, cytochrome C, caspase3, caspase9, p53 and Bax in liver and kidney decreased. Therefore, SCBPE could reduce the damage of liver and kidney caused by CP by reducing the level of oxidative stress, and improving the antioxidant, anti-inflammatory and anti-apoptotic capacity of the body.

Keywords: Apoptotic; Cisplatin toxicity; Inflammation; Kidney injury; Liver injury; Oxidative stress; Schisandra chinensis bee pollen extract.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology
  • Antineoplastic Agents / toxicity
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology
  • Ascorbic Acid / pharmacology
  • Bees
  • Chemical and Drug Induced Liver Injury / etiology
  • Chemical and Drug Induced Liver Injury / prevention & control*
  • Cisplatin / toxicity*
  • Dose-Response Relationship, Drug
  • Free Radical Scavengers / administration & dosage
  • Free Radical Scavengers / pharmacology
  • Kidney Diseases / chemically induced
  • Kidney Diseases / prevention & control*
  • Lipid Peroxidation / drug effects
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects
  • Pollen / chemistry*
  • Rats
  • Rats, Sprague-Dawley
  • Schisandra / chemistry*


  • Anti-Inflammatory Agents
  • Antineoplastic Agents
  • Antioxidants
  • Free Radical Scavengers
  • Malondialdehyde
  • Ascorbic Acid
  • Cisplatin