First Draft Genome Sequence of the Pathogenic Fungus Lomentospora prolificans (Formerly Scedosporium prolificans)

G3 (Bethesda). 2017 Nov 6;7(11):3831-3836. doi: 10.1534/g3.117.300107.

Abstract

Here we describe the sequencing and assembly of the pathogenic fungus Lomentospora prolificans using a combination of short, highly accurate Illumina reads and additional coverage in very long Oxford Nanopore reads. The resulting assembly is highly contiguous, containing a total of 37,627,092 bp with over 98% of the sequence in just 26 scaffolds. Annotation identified 8896 protein-coding genes. Pulsed-field gel analysis suggests that this organism contains at least 7 and possibly 11 chromosomes, the two longest of which have sizes corresponding closely to the sizes of the longest scaffolds, at 6.6 and 5.7 Mb.

Keywords: fungal genomics; genome assembly; nanopore sequencing; pathogen genomics.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Fungal Proteins / genetics
  • Genome, Fungal*
  • Molecular Sequence Annotation*
  • Scedosporium / genetics*
  • Whole Genome Sequencing

Substances

  • Fungal Proteins