Complete Genome Sequences of Human Immunodeficiency Type 1 Viruses Genetically Engineered To Be Tropic for Rhesus Macaques

Masako Nomaguchi; Naoya Doi; Takaaki Koma; Akio Adachi

doi:10.1128/genomeA.01063-17

Complete Genome Sequences of Human Immunodeficiency Type 1 Viruses Genetically Engineered To Be Tropic for Rhesus Macaques

Genome Announc. 2017 Sep 28;5(39):e01063-17. doi: 10.1128/genomeA.01063-17.

Authors

Masako Nomaguchi¹, Naoya Doi¹, Takaaki Koma¹, Akio Adachi^{2

3}

Affiliations

¹ Department of Microbiology, Tokushima University Graduate School of Medical Science, Tokushima, Japan.
² Department of Microbiology, Tokushima University Graduate School of Medical Science, Tokushima, Japan adachi@tokushima-u.ac.jp.
³ Department of Microbiology, Kansai Medical University, Osaka, Japan.

Abstract

We have constructed two human immunodeficiency type 1 (HIV-1) derivatives, CXCR4 tropic and CCR5 tropic, that replicate in rhesus macaques. They are genetically engineered to be resistant to macaque restriction factors against HIV-1, including TRIM5α, APOBEC3, and tetherin proteins. The two HIV-1 variants described here are fundamental clones aiming for rhesus infection studies of HIV-1.