Healing of two-wall intra-bony defects treated with a novel EMD-liquid-A pre-clinical study in monkeys

J Clin Periodontol. 2017 Dec;44(12):1264-1273. doi: 10.1111/jcpe.12825. Epub 2017 Nov 10.


Aim: To investigate the effect of a novel enamel matrix derivative formulation (EMD-liquid or Osteogain) combined with an absorbable collagen sponge (ACS) on periodontal wound healing in intra-bony defects in monkeys.

Materials and methods: Chronic two-wall intra-bony defects were created at the distal aspect of eight teeth in three monkeys (Macaca fascicularis). The 24 defects were randomly assigned to one of the following treatments: (i) open flap debridement (OFD) + ACS alone, (ii) OFD + Emdogain + ACS (Emdogain/ACS), (iii) OFD + Osteogain + ACS (Osteogain/ACS) or (iv) OFD alone. At 4 months, the animals were euthanized for histologic evaluation.

Results: Osteogain/ACS resulted in more consistent formation of cementum, periodontal ligament and bone with limited epithelial proliferation compared to OFD alone, Emdogain/ACS and OFD + ACS. Among the four treatment groups, the Osteogain/ACS group demonstrated the highest amount of regenerated tissues. However, complete periodontal regeneration was not observed in any of the defects in the four groups.

Conclusions: The present findings indicate that in two-wall intra-bony defects, reconstructive surgery with Osteogain/ACS appears to be a promising novel approach for facilitating periodontal wound healing/regeneration, thus warranting further clinical testing.

Keywords: animal study; carrier; enamel matrix proteins; intra-bony defect; periodontal regeneration.

MeSH terms

  • Alveolar Bone Loss / surgery
  • Alveolar Bone Loss / therapy*
  • Animals
  • Bone Regeneration / drug effects
  • Collagen / therapeutic use*
  • Debridement
  • Dental Cementum / pathology
  • Dental Enamel Proteins / therapeutic use*
  • Guided Tissue Regeneration, Periodontal / methods*
  • Haplorhini
  • Macaca fascicularis
  • Male
  • Models, Animal
  • Periodontal Ligament / pathology
  • Periodontal Ligament / surgery
  • Root Planing
  • Wound Healing / drug effects*


  • Dental Enamel Proteins
  • Osteogain
  • enamel matrix proteins
  • Collagen