Integrated Molecular Meta-Analysis of 1,000 Pediatric High-Grade and Diffuse Intrinsic Pontine Glioma

Cancer Cell. 2017 Oct 9;32(4):520-537.e5. doi: 10.1016/j.ccell.2017.08.017. Epub 2017 Sep 28.

Abstract

We collated data from 157 unpublished cases of pediatric high-grade glioma and diffuse intrinsic pontine glioma and 20 publicly available datasets in an integrated analysis of >1,000 cases. We identified co-segregating mutations in histone-mutant subgroups including loss of FBXW7 in H3.3G34R/V, TOP3A rearrangements in H3.3K27M, and BCOR mutations in H3.1K27M. Histone wild-type subgroups are refined by the presence of key oncogenic events or methylation profiles more closely resembling lower-grade tumors. Genomic aberrations increase with age, highlighting the infant population as biologically and clinically distinct. Uncommon pathway dysregulation is seen in small subsets of tumors, further defining the molecular diversity of the disease, opening up avenues for biological study and providing a basis for functionally defined future treatment stratification.

Keywords: DIPG; exome; genome; glioblastoma; histone; methylation.

Publication types

  • Meta-Analysis

MeSH terms

  • Adolescent
  • Brain Stem Neoplasms / genetics*
  • Brain Stem Neoplasms / pathology
  • Cell Cycle Proteins / genetics
  • Child
  • Child, Preschool
  • DNA Topoisomerases, Type I / genetics
  • Exome
  • F-Box Proteins / genetics
  • F-Box-WD Repeat-Containing Protein 7
  • Female
  • Gene Dosage
  • Glioma / genetics*
  • Glioma / pathology
  • Histones / genetics*
  • Humans
  • Infant
  • Infant, Newborn
  • Male
  • Mutation*
  • Proto-Oncogene Proteins / genetics
  • Repressor Proteins / genetics
  • Ubiquitin-Protein Ligases / genetics
  • Young Adult

Substances

  • BCOR protein, human
  • Cell Cycle Proteins
  • F-Box Proteins
  • F-Box-WD Repeat-Containing Protein 7
  • FBXW7 protein, human
  • Histones
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Ubiquitin-Protein Ligases
  • DNA Topoisomerases, Type I