Effect of red light on optically trapped spermatozoa

Biomed Opt Express. 2017 Aug 23;8(9):4200-4205. doi: 10.1364/BOE.8.004200. eCollection 2017 Sep 1.


Successful artificial insemination relies on the use of high quality spermatozoa. One measure of sperm quality is swimming force. Increased swimming force has been correlated with higher sperm swimming speeds and improved reproductive success. It is hypothesized that by increasing sperm swimming speed, one can increase swimming force. Previous studies have shown that red light irradiation causes an increase in sperm swimming speed. In the current study, 633nm red light irradiation is shown to increase mean squared displacement in trapped sperm. The methodology allows for comparison of relative swimming forces between irradiated and non-irradiated samples.

Keywords: (000.1430) Biology and medicine; (350.4855) Optical tweezers or optical manipulation.