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, 11 (8), KC01-KC05

Anti-Alzheimer Properties of Probiotic, Lactobacillus plantarum MTCC 1325 in Alzheimer's Disease Induced Albino Rats

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Anti-Alzheimer Properties of Probiotic, Lactobacillus plantarum MTCC 1325 in Alzheimer's Disease Induced Albino Rats

Mallikarjuna Nimgampalle et al. J Clin Diagn Res.

Abstract

Introduction: Alzheimer's disease is a type of dementia, and till now there is no suitable drug available for the complete cure of this disease. Now-a-days Probiotics, Lactobacillus strains play a therapeutic role in cognitive disorders through Gut-Brain Axis communication.

Aim: The present study was aimed to evaluate the anti-Alzheimer properties of Lactobacillus plantarum MTCC1325 against D-Galactose-induced Alzheimer's disease in albino rats.

Materials and methods: Healthy rats (48) of wistar strain were divided into four groups viz., Group-I: control rats received saline, Group-II: rats received intraperitoneal injection of D-Galactose (120 mg/kg body weight) throughout experiment, Group-III: initially animals were subjected to D-Galactose injection for six weeks, then followed by simultaneously received both D-Galactose and L. plantarum MTCC1325 (12×108 CFU/ml; 10 ml/kg body weight) for 60 days and Group-IV: rats which were orally administered only with Lactobacillus plantarum MTCC1325 for 60 days. During the experimentation, both morphometric and behavioural aspects were studied. Later we have examined histopathological changes and estimated cholinergic levels in selected brain regions of all experimental groups of rats including control on selected days.

Results: Morphometric, behavioural changes, ACh levels were significantly decreased and pathological hallmarks such as amyloid plaques and tangles were also observed in AD model group. Treatment of AD-group with L. plantarum MTCC1325 for 60 days, not only ameliorated cognition deficits but also restored ACh and the histopathological features to control group. However, no significant effects have been observed in the group treated with L. plantarum alone.

Conclusion: The study revealed that, L. plantarum MTCC1325 might have anti-Alzheimer properties against D-Galactose induced Alzheimer's disease.

Keywords: Behavioural activity; Histopathology and cholinergic system; L. plantarum.

Figures

[Table/Fig-1]:
[Table/Fig-1]:
Effect of L. plantarum MTCC 1325 on body weight of experimental and control groups of rats. Values are mean ± SEM (n=6). *p<0.05 vs. Control; #p<0.05 vs. AD model group.
[Table/Fig-3]:
[Table/Fig-3]:
Effect of L. plantarum MTCC 1325 on cognitive performance on selected days in different groups of experimental rats against the control. Values are mean±SEM (n=6). *p<0.05 vs. control; #p<0.05 vs. AD model group.
[Table/Fig-4]:
[Table/Fig-4]:
Effect of Lactobacillus plantarum MTCC 1325 on gross behavioural activity on selected days in different groups of experimental rats against the control. Values are mean±SEM (n=6). *p<0.05 vs. Control; #p<0.05 vs. AD model group.
[Table/Fig-5]:
[Table/Fig-5]:
Sections of cerebral cortex and hippocampus regions of brain from different experimental groups of rats against the control for 30 days treatment. (Congo Red Stain, Light Microscope: 40X). {AD CC: AD-model group Cerebral Cortex; AD+L.P CC: Protective group Cerebral Cotex; L.P CC: Lactobacillus plantarum Group Crerebral Cortex, AD HP: AD-Model group Hippocampus; AD+L.P HP: Protective group Hippocampus; L.P: Lactobacillus plantarum group Hippocampus}.
[Table/Fig-6]:
[Table/Fig-6]:
Sections of Cerebral Cortex and Hippocampus regions of the brain from different experimental groups of rats against the control for 60 days treatment. (Congo Red Stain, Light Microscope: 40X). {AD CC: AD-model group Cerebral Cortex; AD+L.P CC: Protective group Cerebral Cotex; L.P CC: Lactobacillus plantarum Group Crerebral Cortex, AD HP: AD-Model group Hippocampus; AD+L.P HP: Protective group Hippocampus; L.P: Lactobacillus plantarum group Hippocampus}.
[Table/Fig-7]:
[Table/Fig-7]:
Effect of Lactobacillus plantarum MTCC 1325 on the levels of acetylcholine in hippocampus and cerebral cortex regions of control and experimental groups of rats at selected intervals. Values are mean±SEM (n=6). *p<0.05 vs. Control; #p<0.05 vs. AD model group.
[Table/Fig-8]:
[Table/Fig-8]:
Effect of Lactobacillus plantarum MTCC 1325 on the levels of Acetylcholinesterase in hippocampus and cerebral cortex regions of control and experimental groups of rats at selected intervals. Values are mean±SEM (n=6). *p<0.05 vs. Control; #p<0.05 vs. AD model group.

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