Characterization of conserved circular RNA in polyploid Gossypium species and their ancestors

FEBS Lett. 2017 Nov;591(21):3660-3669. doi: 10.1002/1873-3468.12868. Epub 2017 Oct 13.


Circular RNA (circRNA) is a regulatory class of long, noncoding RNA found in both plant and animal kingdoms. The profile and characterization of circRNA in cotton species remains to be explored. Here, using 24 rRNA-depleted RNA-seq libraries of putative diploid progenitors of Gossypium spp., Gossypium arboreum and Gossypium raimondii, their interspecies hybrid (F1 ) and allotetraploid Gossypium hirsutum, 1041, 1478, 1311, and 499 circRNAs were identified in each cotton species, respectively. A prevalence of 23 exon-circRNAs contain noncanonical GT/AG signals, and only ~ 10% of exon-circRNA is associated with reverse complementary intronic sequences. This result implies that plants employ a method of circRNA splicing distinct from that of animals. In addition, 432 circRNAs are stably expressed in multiple cotton species.

Keywords: circRNA; conserved; cotton; polyploidization.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Evolution, Molecular*
  • Gene Expression Regulation / physiology*
  • Gossypium* / genetics
  • Gossypium* / metabolism
  • Polyploidy*
  • RNA, Long Noncoding* / biosynthesis
  • RNA, Long Noncoding* / genetics
  • RNA, Plant* / biosynthesis
  • RNA, Plant* / genetics
  • Species Specificity


  • RNA, Long Noncoding
  • RNA, Plant

Associated data

  • figshare/10.6084/m9.figshare.5484310.v1