Anthocyanin Cyanidin-3-Glucoside Attenuates Platelet Granule Release in Mice Fed High-Fat Diets

J Nutr Sci Vitaminol (Tokyo). 2017;63(4):237-243. doi: 10.3177/jnsv.63.237.

Abstract

Platelet granule release is considered an important target for preventing and treating cardiovascular diseases (CVDs). Cyanidin-3-glucoside (Cy-3-g) is a predominant bioactive anthocyanin compound in many edible plants and has been reported to be protective against CVDs by attenuating platelet dysfunction. However, direct evidence of the action of Cy-3-g on platelet granule secretion in purified platelets from in vivo assays is still poor. In the present study, we demonstrated that dietary supplementation of purified Cy-3-g reduces serum lipid levels and facilitates down-regulation of the platelet granule release of substances such as P-selectin, CD40L, 5-HT, RANTES and TGF-β1 in gel-filtered platelets, in addition to attenuating serum PF4 and β-TG levels in mice fed high-fat diets. These results provide evidence that Cy-3-g protects against thrombosis and CVDs by inhibiting purified platelet granule release in vivo.

Keywords: anthocyanins; cardiovascular diseases; granule release; platelet.

MeSH terms

  • Animals
  • Anthocyanins / pharmacology*
  • Blood Platelets / drug effects
  • Blood Platelets / ultrastructure*
  • Cardiovascular Diseases / prevention & control
  • Chemokine CCL5
  • Diet
  • Diet, High-Fat*
  • Dietary Supplements
  • Glucosides / pharmacology*
  • Lipids / blood
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Platelet Activation / drug effects
  • Platelet Factor 4 / blood
  • Secretory Vesicles / drug effects*
  • Secretory Vesicles / metabolism*
  • Serotonin / blood
  • Transforming Growth Factor beta1 / blood
  • beta-Thromboglobulin / analysis

Substances

  • Anthocyanins
  • Chemokine CCL5
  • Glucosides
  • Lipids
  • Transforming Growth Factor beta1
  • beta-Thromboglobulin
  • cyanidin-3-O-beta-glucopyranoside
  • Serotonin
  • Platelet Factor 4