Isotope-coded protein label based quantitative proteomic analysis reveals significant up-regulation of apolipoprotein A1 and ovotransferrin in the myopic chick vitreous

Sci Rep. 2017 Oct 4;7(1):12649. doi: 10.1038/s41598-017-12650-7.


This study used isotope-coded protein label (ICPL) quantitative proteomics and bioinformatics analysis to examine changes in vitreous protein content and associated pathways during lens-induced eye growth. First, the vitreous protein profile of normal 7-day old chicks was characterized by nano-liquid chromatography electrospray ionization tandem mass spectrometry. A total of 341 unique proteins were identified. Next, myopia and hyperopia were induced in the same chick by attaching -10D lenses to the right eye and +10D lenses to the left eye, for 3 and 7 days. Protein expression in lens-induced ametropic eyes was analyzed using the ICPL approach coupled to LCMS. Four proteins (cystatin, apolipoprotein A1, ovotransferrin, and purpurin) were significantly up-regulated in the vitreous after 3 days of wearing -10D lenses relative to +10D lens contralateral eyes. The differences in protein expression were less pronounced after 7 days when the eyes approached full compensation. In a different group of chicks, western blot confirmed the up-regulation of apolipoprotein A1 and ovotransferrin in the myopic vitreous relative to both contralateral lens-free eyes and hyperopic eyes in separate animals wearing +10D lenses. Bioinformatics analysis suggested oxidative stress and lipid metabolism as pathways involved in compensated ocular elongation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anthraquinones / chemistry
  • Anthraquinones / isolation & purification
  • Apolipoprotein A-I / genetics
  • Apolipoprotein A-I / isolation & purification
  • Chickens
  • Conalbumin / genetics
  • Conalbumin / isolation & purification
  • Cystatins / chemistry
  • Cystatins / isolation & purification
  • Eye / metabolism
  • Eye / physiopathology
  • Hyperopia / genetics*
  • Hyperopia / pathology
  • Hyperopia / veterinary
  • Isotope Labeling
  • Lenses / adverse effects
  • Myopia / genetics*
  • Myopia / pathology
  • Myopia / veterinary
  • Poultry Diseases / genetics
  • Proteomics*
  • Spectrometry, Mass, Electrospray Ionization
  • Vitreous Body / chemistry
  • Vitreous Body / metabolism*
  • Vitreous Body / pathology


  • Anthraquinones
  • Apolipoprotein A-I
  • Cystatins
  • Conalbumin
  • purpurin