The effect of vacuum packaging on histamine changes of milkfish sticks at various storage temperatures

J Food Drug Anal. 2017 Oct;25(4):812-818. doi: 10.1016/j.jfda.2016.12.009. Epub 2017 Feb 14.


The effects of polyethylene packaging (PEP) (in air) and vacuum packaging (VP) on the histamine related quality of milkfish sticks stored at different temperatures (-20°C, 4°C, 15°C, and 25°C) were studied. The results showed that the aerobic plate count (APC), pH, total volatile basic nitrogen (TVBN), and histamine contents increased as storage time increased when the PEP and VP samples were stored at 25°C. At below 15°C, the APC, TVBN, pH, and histamine levels in PEP and VP samples were retarded, but the VP samples had considerably lower levels of APC, TVBN, and histamine than PEP samples. Once the frozen fish samples stored at -20°C for 2 months were thawed and stored at 25°C, VP retarded the increase of histamine in milkfish sticks as compared to PEP. In summary, this result suggested the milkfish sticks packed with VP and stored below 4°C could prevent deterioration of product quality and extend shelf-life.

Keywords: histamine; milkfish stick; total volatile basic nitrogen; vacuum packaging.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Consumer Product Safety
  • Fish Products / analysis*
  • Fishes
  • Food Packaging / instrumentation
  • Food Packaging / methods*
  • Food Storage
  • Histamine / analysis*
  • Humans
  • Temperature
  • Vacuum


  • Histamine

Grants and funding

The study was supported by the Ministry of Science and Technology, R.O.C. (Contract Number MOST 105-2221-E-127-006).