Egg white-derived peptides prevent cardiovascular disorders induced by mercury in rats: Role of angiotensin-converting enzyme (ACE) and NADPH oxidase

Toxicol Lett. 2017 Nov 5;281:158-174. doi: 10.1016/j.toxlet.2017.10.001. Epub 2017 Oct 5.

Abstract

The study aimed to investigate the effects of egg white hydrolysate (EWH) on vascular disorders induced by mercury (Hg). For this, male Wistar rats were treated for 60days: Untreated (saline, i.m.); Mercury (HgCl2, i.m., 1st dose 4.6μg/kg, subsequent doses 0.07μg/kg/day); Hydrolysate (EWH, gavage, 1g/kg/day); Hydrolysate-Mercury. Systolic (SBP) and diastolic (DBP) blood pressure measurement and vascular reactivity experiments in aorta were performed. We analyzed endothelial dependent and independent vasodilator responses and vasoconstrictor response to phenylephrine (Phe) in absence and presence of endothelium, a NOS inhibitor, a NADPH oxidase inhibitor, the superoxide dismutase, a non-selective COX inhibitor, a selective COX-2 inhibitor, an AT-1 receptors blocker. In situ superoxide anion production, SOD-1, NOX-4, p22phox, COX-2 and AT-1 mRNA levels and NOX-1 protein expression were performed in aorta while the determination of angiotensin converting enzyme (ACE) activity was measured in plasma. As results, EWH prevented the increase in SBP and Phe responses and the endothelial dysfunction elicited by Hg, which was related to decreased ACE activity and NOX activation by EWH and, subsequently, alleviated ROS production and improved NO bioavailability in aorta. In conclusion, EWH could be considered as alternative or complementary treatment tools for Hg-induced cardiovascular damage.

Keywords: Angiotensin-converting enzyme (ACE); Blood pressure; Egg white hydrolysate; Mercury; Oxidative stress; Vascular dysfunction.

MeSH terms

  • Animals
  • Aorta / drug effects
  • Aorta / metabolism
  • Blood Pressure / drug effects
  • Cardiovascular Diseases / chemically induced
  • Cardiovascular Diseases / drug therapy*
  • Dose-Response Relationship, Drug
  • Egg White / chemistry*
  • Endothelium, Vascular / drug effects
  • Male
  • Mercury / toxicity*
  • NADPH Oxidases / antagonists & inhibitors
  • NADPH Oxidases / blood*
  • Oxidative Stress / drug effects
  • Peptides / pharmacology*
  • Peptidyl-Dipeptidase A / blood*
  • Phenylephrine / pharmacology
  • Rats
  • Rats, Wistar
  • Vasoconstrictor Agents / pharmacology

Substances

  • Peptides
  • Vasoconstrictor Agents
  • Phenylephrine
  • NADPH Oxidases
  • Peptidyl-Dipeptidase A
  • Mercury