Chaotropic ions affect the conformation of quisqualate receptors in rat cortical membranes

J Neurochem. 1988 Aug;51(2):457-61. doi: 10.1111/j.1471-4159.1988.tb01060.x.


Binding of [3H](R,S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ([3H]AMPA) to quisqualate receptors in the presence of SCN- ions produced curvilinear Scatchard plots. Kinetic investigations of [3H]AMPA binding showed that the curvilinearity cannot be explained by assuming binding to two separate binding sites or by considering it due to cooperative interaction. A more likely explanation is that the quisqualate receptors exist in two states, one with high and one with low affinity for [3H]AMPA. Chaotropic ions change the relaxation constant between the two states.

MeSH terms

  • Animals
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / metabolism
  • Cyanates / pharmacology
  • Cyanides / pharmacology*
  • Ibotenic Acid / analogs & derivatives
  • Ibotenic Acid / metabolism
  • Kinetics
  • Membranes / drug effects
  • Membranes / metabolism
  • Protein Conformation / drug effects
  • Rats
  • Receptors, AMPA
  • Receptors, Drug / drug effects*
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid


  • Cyanates
  • Cyanides
  • Receptors, AMPA
  • Receptors, Drug
  • Ibotenic Acid
  • alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid