Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

Nat Methods. 2017 Nov;14(11):1075-1078. doi: 10.1038/nmeth.4461. Epub 2017 Oct 9.


Nearly all single-particle cryo-EM structures resolved to better than 4-Å resolution have been determined using 300-keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain reconstructions of macromolecular complexes of different sizes to better than 3-Å resolution using a 200-keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules.

MeSH terms

  • Cryoelectron Microscopy / methods*
  • Limit of Detection
  • Macromolecular Substances / chemistry*


  • Macromolecular Substances