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. 2017 Nov 1;65(43):9428-9434.
doi: 10.1021/acs.jafc.7b03410. Epub 2017 Oct 23.

Adsorption of Insecticidal Crystal Protein Cry11Aa Onto Nano-Mg(OH) 2: Effects on Bioactivity and Anti-Ultraviolet Ability

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Adsorption of Insecticidal Crystal Protein Cry11Aa Onto Nano-Mg(OH) 2: Effects on Bioactivity and Anti-Ultraviolet Ability

Xiaohong Pan et al. J Agric Food Chem. .

Abstract

The traditional Bacillus thuringiensis (Bt) formulations for field applications are not resistant to harsh environmental conditions. Hence, the active ingredients of the Bt bioinsecticides could degrade quickly and has low anti-ultraviolet ability in the field, which significantly limits its practical application. In the present study, we developed an efficient and stable delivery system for Bt Cry11Aa toxins. We coated Cry11Aa proteins with Mg(OH)2 nanoparticles (MHNPs), and then assessed the effects of MHNPs on bioactivity and anti-ultraviolet ability of the Cry11Aa proteins. Our results indicated that MHNPs, like "coating clothes", could effectively protect the Cry protein and enhance the insecticidal bioactivity after UV radiation (the degradation rate was decreased from 64.29% to 16.67%). In addtion, MHNPs could improve the proteolysis of Cry11Aa in the midgut and aggravate the damage of the Cry protein to the gut epithelial cells, leading to increased insecticidal activity against Culex quinquefasciatus. Our results revealed that MHNPs, as an excellent nanocarrier, could substantially improve the insecticidal bioactivity and anti-ultraviolet ability of Cry11Aa.

Keywords: Cry11Aa; anti-ultraviolet; bioactivity; nano-Mg(OH)2; proteolysis.

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