Effect of Adalimumab on Peripheral Blood Mononuclear Cells in Non-Infectious Uveitis

Ocul Immunol Inflamm. 2019;27(2):330-337. doi: 10.1080/09273948.2017.1374415. Epub 2017 Oct 11.

Abstract

Purpose: This study analyzed the effect of adalimumab on peripheral blood mononuclear cells (PBMCs) in uveitis.

Methods: PBMCs and serum S100A12 levels from 14 uveitis patients and 28 healthy controls were analyzed. Patient samples were taken before (w0), and 6 (w6) and 12 (w12) weeks after initiation of adalimumab therapy.

Results: Monocytes expressing CD124, CD86, CD39, CD115, and MHCII were decreased in patients. Adalimumab induced CD86+ and CD39+ monocytes, and further decreased the frequency of MHCII- and CD124-positive cells. Patients (w0) had increased percentages of Th1-, Th17-, and Th2 cells and T cell subsets showed a pro-inflammatory polarization (p = 0.02 ratio Th17/Treg patients w0 vs controls), which was reduced upon adalimumab treatment (p = 0.05 w0 vs w6). S100A12 levels were increased in patients (p = 0.02) and reduced under treatment (p = 0.02 for w6/w12).

Conclusions: The phenotype of PBMCs from uveitis patients is modified upon adalimumab treatment. Serum S100A12 levels reflect the systemic immune response.

Keywords: Monocytes; S100A12; T cells; TNFα; uveitis.

MeSH terms

  • Adalimumab / administration & dosage*
  • Adolescent
  • Anti-Inflammatory Agents / administration & dosage
  • Child
  • Child, Preschool
  • Dose-Response Relationship, Drug
  • Female
  • Humans
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / pathology*
  • Male
  • Monocytes / drug effects
  • Monocytes / immunology
  • Monocytes / pathology*
  • Retrospective Studies
  • T-Lymphocytes, Regulatory / drug effects
  • T-Lymphocytes, Regulatory / immunology
  • T-Lymphocytes, Regulatory / pathology*
  • Treatment Outcome
  • Uveitis / blood
  • Uveitis / drug therapy*
  • Uveitis / immunology

Substances

  • Anti-Inflammatory Agents
  • Adalimumab