Techniques to measure DNA single-strand breaks in cells: a review

Int J Radiat Biol. 1988 Nov;54(5):695-707. doi: 10.1080/09553008814552151.


Alkaline sucrose sedimentation was for a number of years the standard procedure for the measurement of single-strand breaks. Some years ago a number of new techniques with improved sensitivity were introduced. The following techniques are presented and discussed: alkaline unwinding, alkaline filter elution, nucleoid sedimentation, viscoelastometry, microelectrophoresis of single cells, DNA precipitation, pulse field gel electrophoresis, fluctuation spectroscopy and nick translation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • DNA Damage*
  • DNA, Single-Stranded*
  • Genetic Techniques


  • DNA, Single-Stranded