3D printing hydrogel with graphene oxide is functional in cartilage protection by influencing the signal pathway of Rank/Rankl/OPG

Zhong Cheng; Bolaky Landish; Zhang Chi; Cui Nannan; Du Jingyu; Lu Sen; Lin Xiangjin

doi:10.1016/j.msec.2017.08.069

3D printing hydrogel with graphene oxide is functional in cartilage protection by influencing the signal pathway of Rank/Rankl/OPG

Mater Sci Eng C Mater Biol Appl. 2018 Jan 1:82:244-252. doi: 10.1016/j.msec.2017.08.069. Epub 2017 Aug 17.

Authors

Zhong Cheng¹, Bolaky Landish², Zhang Chi³, Cui Nannan⁴, Du Jingyu⁵, Lu Sen⁶, Lin Xiangjin⁷

Affiliations

¹ Department of Orthopaedic Orthopaedics, First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China; The Sport Medicine Center of the First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: zhongcheng@zju.edu.cn.
² Department of Orthopaedic Orthopaedics, First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China; The Sport Medicine Center of the First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: landish2009@live.com.
³ Department of Orthopaedic Orthopaedics, First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: locuszc@126.com.
⁴ Department of the Surgical Intensive Care Unit of the First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: zyyycnn@outlook.com.
⁵ The Sport Medicine Center of the First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: About1119@126.com.
⁶ Department of colorectal surgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: Freealex2008@126.com.
⁷ Department of Orthopaedic Orthopaedics, First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China; The Sport Medicine Center of the First Affiliated Hospital, College of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, People's Republic of China. Electronic address: zdyygk@126.com.

PMID: 29025654
DOI: 10.1016/j.msec.2017.08.069

Abstract

3Dprinting is defined as the use of printing technology to deposit living cells, and biomaterials on a given /a substrate. Graphene oxide nanoparticles (GO-np) have been used as a delivery vehicle for small molecule drugs in order to investigate the state of GO-np within 3D tissue constructs in terms of a composite 3D printing scaffold, which in turn is relevant to the protection of cartilage. We transplanted rats with hydrogel/GO-np and hydrogel, which in turn showed that hydrogel/GO-np protected the tissue of cartilage by the signal pathway of Rank/Rankl/OPG. Those findings indicated that GO-np may be potentially used to control the release of carrier materials and influence the signal pathway of Rank/Rankl/OPG.

Keywords: 3D printing; Chondrocytes; Graphene oxide; Rank.

MeSH terms

Animals
Biocompatible Materials / chemistry*
Biocompatible Materials / pharmacology
Bone Morphogenetic Protein 7 / chemistry
Bone Morphogenetic Protein 7 / metabolism
Bone Morphogenetic Protein 7 / pharmacology
Cartilage / drug effects
Cartilage / metabolism*
Cell Line
Cell Survival / drug effects
Chondrocytes / cytology
Chondrocytes / drug effects
Chondrocytes / metabolism
Drug Carriers / chemistry
Graphite / chemistry*
Humans
Hydrogels / chemistry*
Male
Nanoparticles / chemistry
Osteoprotegerin / metabolism*
Oxides / chemistry
RANK Ligand / metabolism*
Rats
Rats, Sprague-Dawley
Receptor Activator of Nuclear Factor-kappa B / metabolism*
Signal Transduction / drug effects
Tissue Scaffolds / chemistry

Substances

Biocompatible Materials
Bone Morphogenetic Protein 7
Drug Carriers
Hydrogels
Osteoprotegerin
Oxides
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Graphite