Immunocytochemical detection of a resistance-associated glycoprotein in tissue culture cells, ascites tumors and human tumor xenografts by Mab 265/F4

Anticancer Res. Jul-Aug 1988;8(4):531-5.

Abstract

The aim of this investigation was to find out whether resistant cells of different tumors can be detected immunocytochemically by the streptavidin-biotin-peroxidase-complex method using the monoclonal antibody 265/F4. This antibody was prepared against the membrane P-glycoprotein of Mr 170 kd from colchicine-resistant CHO cells. For this purpose the acquired resistance of tissue culture cells, ascites tumors and the acquired and inherent resistance of human lung carcinoma xenografts were analyzed. Doxorubicin-resistant S180 cells, daunorubicin-resistant L1210 cells and vincristine-resistant human epidermoid lung carcinoma xenografts showed an intense positive reaction with the monoclonal antibody. In contrast, no specific immunoreactivity was observed with parental (sensitive) tumor cells. These data could eventually provide a prognostic tool for the detection of resistant human tumor cells.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Animals
  • Antibodies, Monoclonal*
  • Biomarkers, Tumor / analysis*
  • Carcinoma, Squamous Cell / drug therapy
  • Carcinoma, Squamous Cell / pathology*
  • Cell Line
  • Daunorubicin / therapeutic use
  • Doxorubicin / therapeutic use
  • Drug Resistance
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Leukemia L1210 / drug therapy
  • Leukemia L1210 / pathology*
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / pathology*
  • Membrane Glycoproteins / analysis*
  • Membrane Glycoproteins / immunology
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Sarcoma 180 / drug therapy
  • Sarcoma 180 / pathology*
  • Transplantation, Heterologous
  • Vincristine / therapeutic use

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • Membrane Glycoproteins
  • Vincristine
  • Doxorubicin
  • Daunorubicin