Functional expression of brain cholecystokinin and bombesin receptors in Xenopus oocytes

Brain Res. 1988 Aug;464(1):75-9. doi: 10.1016/0169-328x(88)90021-6.

Abstract

Total RNA was extracted from 15-day-old whole rat brains. Microinjection of the RNA into Xenopus laevis oocytes induced electrophysiological responsiveness to cholecystokinin-8 (CCK) and bombesin (BBS) but not to corticotropin-releasing factor (CRF) or somatostatin. The responses to CCK and BBS were similar in shape, time course, and reversal potential to that induced by receptor mediated phospholipid breakdown and that which is induced by intracellular injection of IP3. These responses were not blocked by atropine or by mianserin, did not require extracellular Ca2+ and were completely suppressed by intracellular injection of EGTA.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Arginine Vasopressin / metabolism
  • Bombesin / pharmacology
  • Brain / metabolism*
  • Corticotropin-Releasing Hormone / metabolism
  • Female
  • Membrane Potentials / drug effects
  • Microinjections
  • Oocytes / drug effects
  • Oocytes / metabolism*
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism*
  • Rats
  • Receptors, Bombesin
  • Receptors, Cholecystokinin / drug effects
  • Receptors, Cholecystokinin / metabolism*
  • Receptors, Cholecystokinin / physiology
  • Receptors, Neurotransmitter / drug effects
  • Receptors, Neurotransmitter / metabolism*
  • Receptors, Neurotransmitter / physiology
  • Sincalide / pharmacology
  • Somatostatin / metabolism
  • Xenopus laevis / metabolism

Substances

  • RNA, Messenger
  • Receptors, Bombesin
  • Receptors, Cholecystokinin
  • Receptors, Neurotransmitter
  • Arginine Vasopressin
  • Somatostatin
  • Corticotropin-Releasing Hormone
  • Sincalide
  • Bombesin