Ecm33 is a novel factor involved in efficient glucose uptake for nutrition-responsive TORC1 signaling in yeast

FEBS Lett. 2017 Nov;591(22):3721-3729. doi: 10.1002/1873-3468.12882. Epub 2017 Oct 27.

Abstract

Glucose uptake is crucial for providing both an energy source and a signal that regulates cell proliferation. Therefore, it is important to clarify the mechanisms underlying glucose uptake and its transmission to intracellular signaling pathways. In this study, we searched for a novel regulatory factor involved in glucose-induced signaling by using Saccharomyces cerevisiae as a eukaryotic model. Requirement of the extracellular protein Ecm33 in efficient glucose uptake and full activation of the nutrient-responsive TOR kinase complex 1 (TORC1) signaling pathway is shown. Cells lacking Ecm33 elicit a series of starvation-induced pathways even in the presence of extracellular high glucose concentration. This results in delayed cell proliferation, reduced ATP, induction of autophagy, and dephosphorylation of the TORC1 substrates Atg13 and Sch9.

Keywords: glucose uptake; metabolism; nutrient signal.

Publication types

  • Letter

MeSH terms

  • Adaptor Proteins, Signal Transducing / metabolism
  • Adenosine Triphosphate / metabolism
  • Autophagy
  • Autophagy-Related Proteins / metabolism
  • Cell Proliferation
  • Glucose / metabolism*
  • Membrane Proteins / metabolism*
  • Phosphorylation
  • Protein-Serine-Threonine Kinases / metabolism
  • Saccharomyces cerevisiae / growth & development*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Signal Transduction
  • Transcription Factors / metabolism*

Substances

  • ATG13 protein, S cerevisiae
  • Adaptor Proteins, Signal Transducing
  • Autophagy-Related Proteins
  • ECM33 protein, S cerevisiae
  • Membrane Proteins
  • Saccharomyces cerevisiae Proteins
  • TORC1 protein complex, S cerevisiae
  • Transcription Factors
  • Adenosine Triphosphate
  • Protein-Serine-Threonine Kinases
  • SCH9 protein, S cerevisiae
  • Glucose