Evidence that the Abdominal-B r element function is conferred by a trans-regulatory homeoprotein

EMBO J. 1988 Oct;7(10):3223-31.

Abstract

The Abdominal-B gene is a homeotic gene located in the distal-most region of the bithorax complex (BX-C). Based on complementation analysis it has been proposed that the gene contains two separable genetic elements, called the m and r elements. The r element has a chiefly regulatory function confined to parasegment 14. In a reverse Northern screen of the distal-most BX-C DNA, we found four distinct areas that are transcribed in the embryo. One of the transcripts spans a large genomic region which, upon disruption by rearrangement breakpoints, causes loss of r element function. This transcript is expressed in the early embryo in a single domain apparently corresponding to parasegment 14. We propose that the small homeoprotein encoded by the transcript acts as a trans-regulator to confer r element function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Genes, Homeobox*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • RNA, Messenger / genetics*
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Tissue Distribution
  • Transcription Factors / genetics*

Substances

  • RNA, Messenger
  • Transcription Factors