Novel quinoline incorporating 1,2,4-triazole/oxime hybrids: Synthesis, molecular docking, anti-inflammatory, COX inhibition, ulceroginicity and histopathological investigations

Bioorg Chem. 2017 Dec:75:242-259. doi: 10.1016/j.bioorg.2017.09.018. Epub 2017 Sep 30.

Abstract

A series of novel quinolines incorporating 1,2,4-triazole/oxime hybrids were prepared. They showed remarkable anti-inflammatory activity and exhibited very low incidence of gastric ulceration, compared to indomethacin. Most of the compounds tested showed remarkable inhibition of the COX-1 isozyme, with IC50's ranging from 0.48 to 28µM. Compounds 7c and 9g showed high safety profiles with normal stomach tissue integrity. Docking studies supported the observed in vitro inhibitory activity towards the COX enzymes that may explain their promising anti-inflammatory activity relative to indomethacin. Moreover, differences between the COX-1 and COX-2 isozymes in observed energy scores, as well as in the number of interactions with some of the compounds tested, might predict their higher selectivity towards COX-1 rather than COX-2. Compound 9e was found to inhibit both COXs non-competitively with Ki values of 81µM and 94.6µM.

Keywords: Anti-inflammatory; COX inhibition; Histopathology; Oxime; Quinoline; Ulceroginicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / chemical synthesis
  • Anti-Inflammatory Agents / therapeutic use
  • Anti-Inflammatory Agents / toxicity
  • Binding Sites
  • Catalytic Domain
  • Cyclooxygenase 1 / chemistry
  • Cyclooxygenase 1 / metabolism
  • Cyclooxygenase 2 / chemistry
  • Cyclooxygenase 2 / metabolism
  • Cyclooxygenase Inhibitors / chemical synthesis
  • Cyclooxygenase Inhibitors / pharmacology
  • Edema / chemically induced
  • Edema / drug therapy
  • Enzyme Activation / drug effects
  • Gastric Mucosa / drug effects
  • Gastric Mucosa / pathology
  • Inhibitory Concentration 50
  • Kinetics
  • Liver / drug effects
  • Liver / pathology
  • Molecular Docking Simulation
  • Nitric Oxide / metabolism
  • Oximes / chemistry*
  • Oximes / pharmacology*
  • Quinolines / chemistry*
  • Rats
  • Structure-Activity Relationship
  • Triazoles / chemistry*
  • Triazoles / pharmacology

Substances

  • Anti-Inflammatory Agents
  • Cyclooxygenase Inhibitors
  • Oximes
  • Quinolines
  • Triazoles
  • 1,2,4-triazole
  • Nitric Oxide
  • quinoline
  • Cyclooxygenase 1
  • Cyclooxygenase 2