Titrated extract of Centella asiatica increases hair inductive property through inhibition of STAT signaling pathway in three-dimensional spheroid cultured human dermal papilla cells

Biosci Biotechnol Biochem. 2017 Dec;81(12):2323-2329. doi: 10.1080/09168451.2017.1385383. Epub 2017 Oct 16.


Dermal papilla (DP) is a pivotal part of hair follicle, and the smaller size of the DP is related with the hair loss. In this study, we investigated the effect of titrated extract of Centella asiatica (TECA) on hair growth inductive property on 3D spheroid cultured human DP cells (HDP cells). Significantly increased effect of TECA on cell viability was only shown in 3D sphered HPD cells, not in 2D cultured HDP cells. Also, TECA treatment increased the sphere size of HDP cells. The luciferase activity of STAT reporter genes and the expression of STAT-targeted genes, SOCS1 and SOCS3, were significantly decreased. Also, TECA treatment increased the expression of the hair growth-related signature genes in 3D sphered HDP cells. Furthermore, TECA led to downregulation of the level of phosphorylated STAT proteins in 3D sphered HDP cells. Overall, TECA activates the potential of hair inductive capacity in HDP cells.

Keywords: STAT; cell culture; dermal papilla cells; hair growth; titrated extract of Centella asiatica.

MeSH terms

  • Centella / chemistry*
  • Dermis / cytology*
  • Gene Expression Regulation / drug effects
  • Hair / drug effects*
  • Hair / growth & development*
  • Humans
  • Plant Extracts / pharmacology*
  • Signal Transduction / drug effects*
  • Spheroids, Cellular / cytology
  • Spheroids, Cellular / drug effects*
  • Spheroids, Cellular / metabolism


  • Plant Extracts